gary yellen (Addgene inc)

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Addgene inc gary yellen
Gary Yellen, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gary+yellen/pcDNA3%2E1-Peredox-mCherry+(Plasmid+%2332383)/10__1113_slash_jp288960-89-8-10
Average 93 stars, based on 24 article reviews

gary yellen - by Bioz Stars, 2026-09

93/100 stars

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Plasmid Preparation:

Article Title: Human peroxisomal NAD + /NADH homeostasis is regulated by two independent NAD(H) shuttle systems.
Article Snippet: .. pcDNA3.1-Peredox-mCherry was obtained via Addgene from Gary Yellen (Addgene plasmid # 32383). .. To generate Peredox-PTS1, the stop codon of Peredox was replaced by the carboxy terminus of ACOX3, including its peroxisomal targeting signal (PTS1), preceded by a linker GSGGE (GSGGENKPVIGSLKSKL*) using a site-directed mutagenesis kit (NEB, E0554S) and primers (5’-acaaacctgtcataggaagtctgaaatcgaagctctagagtcgagaagcttaagtttaaaccgc-3’, 5’-acttcctatgacaggtttgttttcacctccggaaccagccttgtacagttcgtccatgccgcc-3’).

Article Title: Pyruvate kinase modulates the link between β-cell fructose metabolism and insulin secretion
Article Snippet: .. GW1-PercevalHR was a gift from Gary Yellen (Addgene plasmid # 49082; http://n2t.net/addgene:49082 ; RRID: Addgene_49082) ( ). .. Krebs-Ringer bicarbonate buffer-HEPES (133.4 mM NaCl, 4.7 mM KCl, 1.2 mM KH 2 PO 4 , 1.2 mM MgSO 4 , 2.5 mM CaCl 2 , 5 mM NaHCO 3 , 10 mM HEPES) supplemented with 0.1% bovine-serum albumin (Sigma-Aldrich, St. Louis, MO, USA, Cat# A6003) and 2.8 mM glucose (2.8G-KRBH) adjusted to pH 7.4 was used in in vitro experiments.

Article Title: CISD2 ensures adequate ER-mitochondrial coupling, critically supporting mitochondrial function in neurons.
Article Snippet: SPLICS Mt-ER Short P2A was a gift from Marisa Brini & Tito Calì (Addgene plasmid # 164108; h t t p : / / n 2 t . n e t / a d d g e n e : 1 6 4 1 0 8; RRID:Addgene_164108). .. GW1-PercevalHR was a gift from Gary Yellen (Addgene plasmid # 49082; h t t p : / / n 2 t . n e t / a d d g e n e : 4 9 0 8 2 ; RRID:Addgene_49082). pCMV CEPIA2mt was a gift from Masamitsu Iino (Addgene plasmid # 58218; http://n2t.net/addgene:58218; RRID:Addgene_58218). ..

Article Title: Subcellular Compartmentalization of Glucose Mediated Insulin Secretion
Article Snippet: .. pGW1CMV-Perceval plasmid, a gift from Gary Yellen (Addgene, Watertown, MA USA, plasmid #49082), was cloned into pShuttleCMV by UCLA Vector Core as described by the Rutter’s group [13]. .. Adenoviral particles were produced by the AdEasy Adenoviral Vector System (Agilent, Santa Clara, CA, USA.

Article Title: CISD2 ensures adequate ER-mitochondrial coupling, critically supporting mitochondrial function in neurons
Article Snippet: SPLICS Mt-ER Short P2A was a gift from Marisa Brini & Tito Calì (Addgene plasmid # 164108; http://n2t.net/addgene:164108 ; RRID:Addgene_164108). .. GW1-PercevalHR was a gift from Gary Yellen (Addgene plasmid # 49082; http://n2t.net/addgene:49082 ; RRID:Addgene_49082). pCMV CEPIA2mt was a gift from Masamitsu Iino (Addgene plasmid # 58218; http://n2t.net/addgene:58218 ; RRID:Addgene_58218). ..

Article Title: Metabolic priming by multiple enzyme systems supports glycolysis, HIF1α stabilisation, and human cancer cell survival in early hypoxia.
Article Snippet: .. The authors also thank Alex Gould and Pia Ballschmieter for critical reading of the manuscript. pMSCV-Peredox-mCherry-NLS was a gift from Gary Yellen (Addgene plasmid # 32385), SpCas9(BB)-2A-Puro (PX459) V2.0 was a gift from Feng Zhang (Addgene plasmid # 62988). pUC57-LbNOX was a gift from Vamsi Mootha (Addgene plasmid # 75285). ..

Article Title: Metabolic priming by multiple enzyme systems supports glycolysis, HIF1α stabilisation, and human cancer cell survival in early hypoxia.
Article Snippet: MCF10A cells were seeded in DMEM/F12 (Generon Cat# L0091) supplemented with 5.5 mM glucose, 5% dialysed horse serum (MWCO 3500), 2 mM L-glutamine, 20 ng/ml EGF (PreproTech), 0.5 μg/ml hydrocortisone, 100 ng/ml cholera toxin, 10 μg/ml insulin and penicillin–streptomycin. .. pMSCV-Peredox-mCherry-NLS (Addgene plasmid # 32385) was a gift from Gary Yellen (Hung et al, 2011); pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene plasmid # 62988) was a gift from Feng Zhang (Ran et al, 2013). .. Plasmids pLenti-HA-IRES-BSD (PS100104) and pLenti-GFP-P2A-BSD (PS100103) were from Origene. pUC57-LbNOX was a gift from Vamsi Mootha (Addgene plasmid # 75285) and used to subclone LbNOX into the MluI and XhoI sites of pLenti-HA-IRES-BSD.

Construct:

Article Title: Lactate potentiates NMDA receptor currents via an intracellular redox mechanism targeting GluN2B subunits: implications for synaptic plasticity
Article Snippet: In addition we obtained a pCMV6-Entry vector expressing CaMKIIα (MR207662, accession NM_177407) from OriGene (Rockville, MD, USA). .. The pcDNA3.1-Peredox mCherry construct was a gift from Gary Yellen (Addgene, Watertown, MA, USA, #32383). .. Multiple point mutants of the Grin2b and CaMKII constructs were generated by GenScript.

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gary yellen (Addgene inc)

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